Among several potentials of nanotechnology applications in food, development of nanoscale sensors for
food safety and security measurement are emerging. A novel bio-functional nanosensor for foodborne
pathogenic bacteria detection was developed using hetero-Au/Si nanorods. For the development of
nanobiosensor, the protocol for bio-functional nanorod fabrication has been developed. The protocols
include; 1) Silicon nanorod fabrication including substrate preparation and deposition control; 2)
Surface oxidation including annealing and oxygen plasma process; 3) Nanoparticle coating onto the
silicon using sputter coating system; 4) Biological dye immobilization including APTES treatment and
incubation process; 5) Antibody conjugation with DSP pretreatment and antibody incubation followed
by antigen/infected cells preparation; finally, 6) Antigen/infected cells detection by bio-functional
nanorods. The Si nanorods were fabricated by glancing angle deposition (GLAD) method, and Au was
sputtered onto the silicon nanorods. Alexa488-succinimide dye molecules were immobilized onto the
annealed silicon nanorods thru the attachment between dye ester and primary amine supplied by
3-Aminopropyltriethoxysilane (APTES). Anti-Salmonella was conjugated to the Au via Dithiobis
[succinimidylpropionate] (DSP) self-assembly monolayer (SAM). Due to the high aspect ratio nature of
Si nanorods, hundreds or thousands of dye molecules attached to silicon nanorods enhanced
fluorescence signals. These biologically functionalized nanorods can be used for nanobiosensor to
detect foodborne pathogenic bacteria with fluorescent microscopic imaging. This new nanoscale
sensing technology will be of great significance for food safety and security applications as well as
biomedical diagnostics.
https://mediaspace.gatech.edu/media/park/1_ytlg21yx
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